Comparison of four functionalization methods of gold nanoparticles for enhancing the enzyme-linked immunosorbent assay (ELISA)

• Paula Ciaurriz,
• Fátima Fernández,
• Edurne Tellechea,
• Jose F. Moran and
• Aaron C. Asensio

Beilstein J. Nanotechnol. 2017, 8, 244–253, doi:10.3762/bjnano.8.27

• functionalization for ELISA enhancement. In this work, we use 20 nm gold nanoparticles (AuNPs) as a vehicle for secondary antibodies and peroxidase (HRP). The design of experiments technique (DOE) and four different methods for biomolecule loading were compared using a rabbit IgG/goat anti-rabbit IgG ELISA model

• approach, our data showed a sensitivity increase of at least five times and a lower detection limit with respect to a standard ELISA of at least three times. Additionally, the assay time was remarkably decreased. Keywords: allergen; ELISA enhancement; functionalization; gliadin; gold nanoparticle

• goat anti-rabbit IgG (Ab) and HRP (AuNPs-Ab-HRP) was assayed to elucidate the best conditions for biomolecule binding and ELISA enhancement. We explored the effect of four different described procedures for binding antibodies and HRP to AuNP surfaces in order to enhance ELISA sensitivity. Afterwards